首页 新闻   会展报道 厂商动态 科技 学术 仪器展厅   产品展示 推荐展商 产品分类 人才 求职 招聘 耗材 ·设为首页
供求 资料   分析方法 仪器资料 论文 标准 实用网址   化学工具 学习资源 化工机构 会展 会议 展览 论坛 ·加入收藏
首 页 新 闻 仪器展厅 耗材配件 供 求 资料中心 人 才 会 展 实用网址 会员中心 论坛
分析方法 | 标准 | 论文集 | 仪器资料 | 用户通讯 | 认证认可 | 相关软件 | 其他资料
   
资料名称

Effect of Linker and Spacer on the Design of a Fib

资料类型 PDF文档
资料大小 608.07 K
类 别 标准
资料来源 Langmuir
简 介 The design of a fibronectin-mimetic peptide that specifically binds to the R51 integrin has been widely studied because of this integrin’s participation in many physiological and pathological processes. A promising design for such a peptide includes both the primary binding site RGD and the synergy site PHSRN connected by a linker and extended off of a surface by a spacer. Our original hypothesis was that the degree of hydrophobicity/hydrophilicity between the two sequences (RGD and PHSRN) in fibronectin is an important parameter in designing a fibronectin-mimetic peptide (Mardilovich, A.; Kokkoli, E. Biomacromolecules 2004, 5, 950-957). A peptide-amphiphile, PR_b, that was previously designed in our laboratory employed a hydrophobic tail connected to the N terminus of a peptide headgroup that was composed of a spacer, the synergy site sequence, a linker mimicking both the distance and hydrophobicity/ hydrophilicity present in the native protein fibronectin (thus presenting an overall “neutral” linker), and finally the primary binding sequence. Even though our previous work (Mardilovich, A.; Craig, J. A.; McCammon, M. Q.; Garg, A.; Kokkoli, E. Langmuir 2006, 22, 3259-3264) demonstrated that PR_b is a promising sequence compared to fibronectin, this is the first study that tests our hypothesis by comparing PR_b to other peptides with hydrophobic or hydrophilic linkers. Furthermore, different peptide-amphiphiles were designed that could be used to study the effect of building blocks systematically, such as the peptide headgroup linker length and hydrophobicity/hydrophilicity as well as the headgroup spacer length on integrin adhesion. Circular dichroism spectroscopy was first employed, and the collected spectra demonstrated that only one peptide-amphiphile exhibited a secondary structure. Their surface topography was evaluated by taking atomic force microscopy (AFM) images of Langmuir-Blodgett peptide-amphiphile membranes supported on mica.
上传人 瑞典百欧林科技有限公司上海代表处
上传时间 2012-12-04
下载次数 0
消耗积分 2
立即下载

**下载后对该资源进行评论,即可获得2个积分。
本栏目部分资源来自互联网,仅为促进数据共享和信息交流,如有版权问题请告知本网或上传者本人,本网会及时给予答复。

报告错误 1.下载无效      2. 资料明显无价值      3. 资料重复存在
下载排行榜
最新上传资料
互联网站: 中国聚合物网  -   中国流变网  -  中国塑料技术网  -   化学化工论坛
- 关于我们 - 网站导航 - 联系我们 - 法律声明 - 友情链接 -
主办:中国化学会
协办:中国化工学会化工新材料专业委员会
            中国塑料加工工业协会塑料技术协作委员会
Chemshow.cn Copyright(C)2006 ,All Right Reserved. 版权所有,未经书面授权,所有页面内容不得以任何形式复制.